Maximizing Photosynthesis-Driven Baeyer–Villiger Oxidation Efficiency in Recombinant Synechocystis sp. PCC6803
نویسندگان
چکیده
Photosynthesis-driven whole-cell biocatalysis has great potential to contribute a sustainable bio-economy since phototrophic cells use light as the only energy source. It yet be shown that microorganisms, such cyanobacteria, can combine supply of high heterologous enzyme levels with allocation sufficient reduction equivalents enable efficient light-driven redox biocatalysis. Here, we demonstrated expression an NADPH-dependent Baeyer–Villiger monooxygenase (BVMO) gene from Acidovorax sp. CHX100 turns Synechocystis PCC6803 into oxyfunctionalization biocatalyst, deriving electrons and O 2 photosynthetic water oxidation. Several systems were systematically tested, P nrsB-(Ni 2+ )–controlled based on replicative plasmid yielded highest intracellular concentration activities up 60.9 ± 1.0 U g CDW ?1 . Detailed analysis reaction parameters, side reactions, biocatalyst durability revealed—on one hand—a in vivo BVMO activity range 6 mg and—on other hand—an impairment performance by product toxicity by-product inhibition. Scale-up 2-L fed-batch photo-bioreactors resulted stabilization bioconversion over several hours maximal specific 30.0 0.3 , volumetric productivity 0.21 0.1 gL h formation 1.3 ? -caprolactone. Process simulations determined kinetic data revealed photosynthesis-driven cyclohexanone oxidation scale under high-light conditions was kinetically controlled not subject limitation photosynthesis.
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ژورنال
عنوان ژورنال: Frontiers in catalysis
سال: 2022
ISSN: ['2673-7841']
DOI: https://doi.org/10.3389/fctls.2021.780474